Mitochondrial Respiratory Chain Enzyme Analysis – Skin Fibroblasts
Method Description Describes how the test is performed and provides a method-specific reference
The respiratory chain enzyme activities are measured at 30 C using a
temperature-controlled spectrophotometer. The activities of complex I
(Ferricyanide:NADH dehydrogenase), complex II (succinate
dehydrogenase), complex I+III (NADH:cytochrome c oxidoreductase),
complex II+III (succinate:cytochrome c reductase) and complex IV
(cytochrome c oxidase) are assayed using different electron acceptors/
donors. The activities of complex I+III and II+III were measured by
following the reduction of cytochrome c at 550 nm, of complex I by
following the oxidation of NADH at 340 nm, of complex II by following
the reduction of 2,6-dichloroindophenol (DCIP) at 600 nm and of complex
IV by following the oxidation of reduced cytochrome c at 550 nm. Values
are normalized to citrate synthase when it exceeds 1 SD from the mean.
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Performing Laboratory Location The location of the laboratory that performs the test
Baylor Medical Genetics Laboratories