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Hexane solvent extraction of saponified lipids followed by derivatization with BSTFA + 1% TMCS. Gas chromatography on a 0.20 mm X 25 m OV-1 (0.33 um phase) capillary column programmed from 160 to 290 degrees C at 4 degrees C per min. Quantification by dual flame ionization detection and selected-ion monitoring, ion-ratio mass fragmentometry in the electron impact mode using epicoprostanol as internal standard.
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