Test ID: BHIS
Bone Histomorphometry, Quantitative
Method Description 
Describes how the test is performed and provides a method-specific reference
Intact (nonfragmented) specimens of undecalcified bone are dehydrated in ethanol, embedded in methylmethacrylate (cold polymerization), sectioned at 5 micron thickness, and individual sections are stained with Goldner's Trichrome, toluidine blue, hematoxylin and eosin. Special stains for acid phosphatase, aluminum, and iron are performed when indicated. Histomorphometry, by computerized technique, is then done on the stained sections using light microscopy and on unstained sections using fluorescence microscopy. Histomorphometric values and the histologic appearance of all sections are reviewed and interpreted by 1 of 7 clinician-histomorphometrists. A hematoxylin-and-eosin-stained section is reviewed for abnormalities by a pathologist. Telephone consultations with responsible physicians are carried out whenever possible. (Hodgson SF, Johnson KA, Muhs JM, et al: Outpatient percutaneous biopsy of the iliac crest: methods, morbidity, and patient acceptance. Mayo Clin Proc 1986;61:28-33)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Friday; 8 a.m. - 5 p.m.
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