Bone Histomorphometry, Consultant Interpretation Only
Method Description Describes how the test is performed and provides a method-specific reference
Intact (nonfragmented) specimens of undecalcified bone are dehydrated in ethanol, embedded in methylmethacrylate (cold polymerization), sectioned at 5-10 micron thickness, and individual sections are stained with Goldner's Trichrome, solochrome, hematoxylin and eosin. Special stains for acid phosphatase, aluminum and iron are obtained when indicated. Histomorphometry, by computerized technique, is then done on the stained sections using light microscopy and on unstained sections using fluorescence microscopy. Histomorphometric values and the histologic appearance of all sections are reviewed and interpreted by one of 4 clinician-histomorphometrists. A hematoxylin-and-eosin-stained section is reviewed for abnormalities by a pathologist.
Telephone consultations with responsible physicians are carried out whenever possible. (Hodgson SF, Johnson KA, Muhs JM, et al: Outpatient percutaneous biopsy of the iliac crest: methods, morbidity, and patient acceptance. Mayo Clin Proc 61:28-33, 1986)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Friday; 8 a.m. - 5 p.m.
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Performing Laboratory Location The location of the laboratory that performs the test