Intact (nonfragmented) specimens of undecalcified bone are dehydrated in ethanol, embedded in methylmethacrylate (cold polymerization), sectioned at 5 to 10 micron thickness, and individual sections are stained with Goldner's Trichrome, solochrome, hematoxylin and eosin. Special stains for acid phosphatase, aluminum, and iron are obtained when indicated. Histomorphometry, by computerized technique, is then done on the stained sections using light microscopy and on unstained sections using fluorescence microscopy. Histomorphometric values and the histologic appearance of all sections are reviewed and interpreted by 1 of 4 clinician-histomorphometrists. A hematoxylin and eosin-stained section is reviewed for abnormalities by a pathologist.
Telephone consultations with responsible physicians are carried out whenever possible.(Hodgson SF, Johnson KA, Muhs JM, et al: Outpatient percutaneous biopsy of the iliac crest: methods, morbidity, and patient acceptance. Mayo Clin Proc 1986;61:28-33)