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Test ID: SPNC    
Streptococcus pneumoniae Antigen, Spinal Fluid

Method Description Describes how the test is performed and provides a method-specific reference

The Binax NOW Streptococcus pneumoniae test is an immunochromatographic membrane assay used to detect pneumococcal-soluble antigen in cerebrospinal fluid. Rabbit anti-Streptococcus pneumoniae and antispecies antibodies are conjugated to visualizing particles that are dried onto an inert fibrous support. The resulting conjugate pad and the striped membrane are combined to construct the test strip.

 

To perform the test, a swab is dipped into the specimen, removed, and then inserted into the test device. Reagent A, a buffer solution, is added from a dropper bottle. The device is then closed, bringing the sample into contact with the test strip. Pneumococcal antigen present in the sample reacts to bind anti-Streptococcus pneumoniae conjugated antibody, forming the sample line. Immobilized control antibody captures antispecies conjugate forming the control line.

 

Test results are interpreted by the presence or absence of visually detectable pink-to-purple colored lines. A positive test result, read in > or =15 minutes depending on the concentration of antigen present in the sample, will include the detection of both a sample and control line. A negative test result, read in 15 minutes, will produce only a control line, indicating that Streptococcus pneumoniae antigen was not detected in the sample. Failure of the control line to appear, whether the sample line is present or not, indicates an invalid assay.(Package insert: NOW Streptococcus pneumoniae Test. Binax, Inc., Portland, ME, 1998)

PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information

No

Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Monday through Sunday; 12 p.m.

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

Same day/1 day

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result

1 day

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

14 days

Performing Laboratory Location The location of the laboratory that performs the test

Rochester