Test ID: SAAS
Sperm Antibody, Direct, Semen
Method Description
Describes how the test is performed and provides a method-specific reference
This assay has been developed on the principle that the antigen(s) is a sperm surface protein(s). Such antigens may induce production of antibody in the person himself or his female partner. Direct testing for the presence of antibodies on sperm is accomplished by incubating washed sperm with the immunobeads. The presence of sperm antibodies is indicated by the binding of the polyacrylamide beads to motile sperm. The proportion of sperm bound and the sites of binding are determined by counting a minimum of 100 cells.(Bronson R, Cooper G, Rosenfeld D: Sperm antibodies: their role in infertility. Fertil Steril 1984;42:171-183)
For specimens collected on-site at Mayo Rochester, if patient's sperm count is <5 million/mL or motility is <10%, indirect testing of seminal plasma may be indicated due to insufficient motile sperm for direct testing.
Day(s) and Time(s) Test Performed
Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Friday, Set up at 8:30 a.m.
Send specimen Monday through Thursday only and not the day before a holiday.


