Fluticasone 17-Beta-Carboxylic Acid, Urine
Method Description Describes how the test is performed and provides a method-specific reference
Fluorometholone (4 ng/mL) internal standard is added to each calibrator, control, and urine sample. Fluticasone 17-beta-carboxylic acid is extracted from 1 mL of urine using an acid based acetonitrile precipitation followed by methylene chloride liquid extraction of the supernatant. 60 uL of the reconstituted sample extract is injected onto a high-performance liquid chromatography system and analyzed by tandem mass spectrometry. The mass spectrometer operates under the electrospray interface and is operated in the multiple-reaction monitoring positive mode. The calibration utilizes an 8-point calibration curve over a concentration range of 0 to 10,000 pg/mL.(Unpublished Mayo method)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Tuesday; 11 a.m.
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location The location of the laboratory that performs the test