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Test ID: 89669    
KIT, Mutation Analysis, Exon 9

Method Description Describes how the test is performed and provides a method-specific reference

The paraffin-embedded tissue is macroscopically examined and the tumor-rich portion is dissected, deparaffinized, lysed, and digested. Genomic DNA is extracted using either a phenol-chloroform method or the QIAamp DNA FFPE Tissue Kit (Qiagen). The DNA is amplified via PCR. Primers specific for KIT exon 9 are used. Negative or wild-type and no template or water controls are used for each run. The patient and control samples are sent for direct DNA sequencing. The sequencing chromatograms are analyzed by manual and software methods and the presence or absence of a KIT exon 9 mutation is determined. The results are interpreted and reported by a working group pathologist.(Qiagen DNA FFPE Tissue Handbook; unpublished Mayo method)

Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Monday through Friday; 8 a.m.-5 p.m.

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

4 days

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result

7 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

1 week/7 days

Performing Laboratory Location The location of the laboratory that performs the test

Rochester