Chromosome Analysis, Spontaneous Breakage Analysis, Blood
Method Description Describes how the test is performed and provides a method-specific reference
In the laboratory, a portion of the whole blood is transferred to a flask containing media with a T-cell mitogen. The cells are incubated for 72 hours at 37 degrees C. In the harvest process, the cells are exposed to colcemid, hypotonic solution, and fixed with glacial acetic acid and methanol. Metaphase cells are dropped onto microscope slides and stained for G-banding with Leischman stain. A concurrent control sample is processed and analyzed. Fifty metaphases from the patient and control are examined to identify chromosome rearrangements. (Unpublished Mayo method)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Samples processed Monday through Sunday. Results reported Monday through Friday, 8 a.m.-5 p.m. CST.
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location The location of the laboratory that performs the test