This test uses a 1-dimensional, thin-layer chromatography (TLC) technique for separating the phospholipids in amniotic fluid. The first step in the process involves extraction of the lipids present in the amniotic fluid using a chloroform/methanol mixture. The extracted specimen is streaked onto a TLC plate and placed in tank 1 for prechromatography, a step that removes a component that otherwise comigrates with PG. Lipids are then separated using tank 2. The separated lipids are made visible with dilute cupric acetate and charring. L to S is determined using a scanning densitometer. The relative intensities of L and S are expressed as a ratio, and PG is reported from a visual observation of the plate and reported as present, absent, or trace.(Unpublished Mayo method)
Testing set-up until 2 pm Monday through Saturday, and 11 am Sunday
Samples received after these times will be run the next day.