Test ID: JAKXB
JAK2 Exon 12 and Other Non-V617F Mutation Detection, Blood
Method Description 
Describes how the test is performed and provides a method-specific reference
Total RNA is extracted from whole blood and cDNA synthesized from JAK2 mRNA. A fragment spanning exons 12 through 15 is then amplified using standard PCR and the sequence obtained using Sanger sequencing (BigDye terminator V1.1 cycle sequencing kit from Applied Bioscience) with analysis on an automated genetic analyzer.(Applied Biosystems 3130; unpublished Mayo method)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Friday
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
5 days
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
8 days
Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
RNA 1 year
Performing Laboratory Location The location of the laboratory that performs the test
Rochester
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