The reverse sequence-specific oligonucleotide (SSO) DNA typing method consists of using PCR to amplify target DNA with group specific primers. The PCR product is denatured and allowed to rehybridize to complementary DNA probes bound to fluorescently coded microspheres. The mixture is labeled with a fluorescent dye that is able to detect biotinylated protein and nucleic acids. A flow analyzer detects the fluorescent emission on each probe and the reaction pattern is analyzed and interpreted. The assignment of the human leukocyte antigen (HLA) typing is based on the reaction pattern compared to patterns associated with published HLA gene sequences. This procedure produces a class I/II HLA low-to-medium resolution typing for use in transplant diagnostics and disease association.(Package insert: LABType SSO Typing Test, Canoga Park, CA: One Lamda, Inc.; Rev 19).
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Whole Blood 7 days; Extracted DNA 2 months