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Test ID: DISI    
HLA Class I Molecular Typing Disease Association

Method Description Describes how the test is performed and provides a method-specific reference

The reverse sequence-specific oligonucleotide (SSO) DNA typing method consists of using PCR to amplify target DNA with group specific primers. The PCR product is denatured and allowed to rehybridize to complementary DNA probes bound to fluorescently coded microspheres. The mixture is labeled with a fluorescent dye that is able to detect biotinylated protein and nucleic acids. A flow analyzer detects the fluorescent emission on each probe and the reaction pattern is analyzed and interpreted. The assignment of the human leukocyte antigen (HLA) typing is based on the reaction pattern compared to patterns associated with published HLA gene sequences. This procedure produces a class I/II HLA low-to-medium resolution typing for use in transplant diagnostics and disease association.(Package insert: LABType SSO Typing Test, Canoga Park, CA: One Lamda, Inc.; Rev 19).

Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Monday through Friday; 7:30 a.m.-5 p.m.

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

5 days

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result

9 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

Whole Blood 7 days; Extracted DNA 2 months

Performing Laboratory Location The location of the laboratory that performs the test

Rochester