Test ID: TACIG
Transmembrane Activator and CAML Interactor (TACI) Gene, Known Mutation Analysis
Method Description
Describes how the test is performed and provides a method-specific reference
Genomic DNA is extracted from whole blood, followed by TACI gene amplification by PCR for the specific exon. The PCR product is separated from unincorporated primers, nucleotides, and contaminants by column purification, then sequenced in both directions using sequencing primers and fluorescent dye-terminator chemistry. The sequencing product is separated on an automated sequencer and trace files analyzed for variations in the specific exon and intron/exon boundaries using specialized mutation detection software and visual inspection (unpublished Mayo method).
Day(s) and Time(s) Test Performed
Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Wednesday
Analytic Time
Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
28 days
Maximum Laboratory Time
Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
42 days
Specimen Retention Time
Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Whole Blood: 2 weeks
Extracted DNA: 2 months
Performing Laboratory Location
The location of the laboratory that performs the test
Rochester


