Test ID: MNB
Manganese, Blood
Method Description
Describes how the test is performed and provides a method-specific reference
Manganese in whole blood is determined by Zeeman background correction Graphite Furnace Atomic Absorption Spectrometry. Aqueous acidic calibrating standards with surfactant are diluted with a whole blood matrix containing normal concentrations of manganese. Reagent blanks are diluted using aqueous acidic calibrating standards with surfactant and reagent grade water in place of whole blood matrix. Quality control specimens and patient samples are diluted in an identical manner. In turn, all diluted blanks, calibrating standards, quality control samples and patient samples are drawn into an auto sampler tube and deposited from the tube onto the platform of the graphite rod within the furnace. The graphite rod with internal platform is placed in the light path from a hollow cathode lamp. When sufficient current is applied to this rod the temperature rises from ambient to 2450 degrees C in less than 5 seconds, atomizing the elements in the sample. Quantification is achieved by measuring the absorbance of the manganese resonance line at 279.5 nm from the hollow cathode lamp. Instrumentation response is defined by the linear relationship of analyte concentration versus the ratio of the absorption signals. After reagent blank subtraction, unknown sample concentrations are calculated by entering the net unknown intensity ratios into the linear calibration equation.(Unpublished Mayo Method)
Day(s) and Time(s) Test Performed
Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Friday; 11 a.m.


