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Unit Code 8905:
Orotic Acid, Urine

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Method Description

Interfering substances such as amino acids, urea, pigments, and

creatinine are removed from the urine by passing it through a

cation-exchange resin.  The orotic acid is then brominated to form

dibromobarbituric acid, reduced to barbituric acid with ascorbic acid

and finally condensed with dimethylaminobenzaldehyde to form

5(p-dimethylaminobenzaldehyde)-barbituric acid. The absorbance

of the final product is measured at 480 nm using a control reaction as

reference.  (Harris ML, Oberholzer VG:  Conditions affecting the

colorimetry of orotic acid and orotidine in urine. Clin Chem

1980;26:473-479)

Performing Laboratory Location

Rochester

Key