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Test ID: 89027    
Ki-67(MIB-1), Quantitative Immunohistochemistry, Automated

Method Description Describes how the test is performed and provides a method-specific reference

A 4-micron thick section is cut from the paraffin block. The section is stained with an immunoperoxidase method using the monoclonal antibody Ki-67 (MIB-1 clone). This is the paraffin nuclear epitope to the Ki-67 antigen. Any nucleus that has an antigen-antibody complex will cause the bright-field, brown chromogen, diaminobenzidine (DAB), to precipitate onto it. All nuclei, both DAB positive and negative, are counterstained with diluted hematoxylin.

 

Ki-67(MIB-1)-stained slides are scanned using the ACIS III Instrument, which captures digital images of the patient slide. Selection of specific areas within the digitalized image for ACIS III scoring is performed by the operator of the ACIS who measures 10 representative areas of the tumor specimen under a 40X field of view. The nuclear-area staining with Ki-67(MIB-1) is reported as a percentage of the total hematoxylin-stained nuclear area. The slides and test results are then reviewed by a pathologist who provides a final interpretation.(Unpublished Mayo method) 

Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Monday through Friday; 8 a.m.-5 p.m.

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

2 days

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result

10 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

Until 1 week after results are reported

Performing Laboratory Location The location of the laboratory that performs the test

Rochester