Viral Culture, Respiratory
Method Description Describes how the test is performed and provides a method-specific reference
Specimens are inoculated into conventional tube cell cultures (MRC-5 and RMK) and observed for the presence of cytopathic effects (CPE) over a 9 to 14 day period. Results of viral cultures are reported when typical CPE are detected and the identity of the isolate has been confirmed by immunofluorescence with specific antisera.(Smith TF: Antibody-enhanced detection of viruses in cell cultures. In Manual of Clinical Laboratory Immunology. Fifth edition. Edited by NR Rose, EC de Macario, JD Folds, et al. Washington, DC, ASM Press, 1997, pp 618-624)
The shell-vial assay is also performed on specimens submitted for cytomegalovirus or herpes simplex virus. Shell-vial results may be positive within 16 to 24 hours postinoculation.(Paya CV, Wold AD, Smith TF: Detection of cytomegalovirus infections in specimens other than urine by the shell vial assay and conventional tube cell cultures. J Clin Microbiol 1987;25:755-757)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Sunday; Varies
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location The location of the laboratory that performs the test