Unit Code 88897:
B-Cell Lymphoma, FISH, Blood or Bone Marrow
Method Description
Anomalies involving BCL6 and c-MYC are detected using a break-apart
FISH probe. If a c-MYC abnormality is detected, reflex testing will be
performed to identify the translocation partner. Translocations involving
fusion of CCND1 and IGH; BCL2 and IGH; c-MYC and IGH; API2 and
MALT1; and IGH and MALT1 are detected using dual-color, dual-fusion
(D-FISH) strategies. A total of 200 nuclei are scored for the break-apart
probe set. For each D-FISH probe set, 500 interphase nuclei are scored
and results for each abnormal probe(s) are expressed as percent
abnormal nuclei. (Brockman SR, Paternoster SF, Ketterling RP, et al:
New highly sensitive fluorescence in situ hybridization method to detect
PML/RARA fusion in acute promyelocytic leukemia. Cancer Genet
Cytogenet 2003;145:144-51)
Performing Laboratory Location
Rochester
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