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Test ID: ROTA    
Rotavirus Antigen, Feces

Method Description Describes how the test is performed and provides a method-specific reference

This FDA-approved kit utilizes monoclonal antibodies in a solid-phase sandwich type EIA. Plastic microtiter wells are coated with a monoclonal antibody directed against the product of the sixth viral gene (VP6), which is the group-specific antigen for all known human rotaviruses. An aliquot of fecal suspension is added to the well and incubated simultaneously with an anti-rotavirus monoclonal antibody conjugated to horseradish peroxidase, resulting in the rotavirus antigen being sandwiched between the solid-phase and enzyme-linked antibodies. After 60 minutes incubation at ambient temperature, the specimen well is washed in order to remove unbound enzyme-labeled antibodies. Enzyme substrate (urea peroxidase) and chromogen tetra methylbenzidine (TMB) are added to the wells and incubated for 10 minutes at ambient temperature. The enzyme bound in the wells converts the colorless substrate to a blue color. The intensity of the blue color is directly proportional to the concentration of rotavirus antigen in the specimen. (Package insert: Premier Rotaclone Steele AD, Williams MM, Bos P, Peenze I: Comparison of two rapid enzyme immunoassays with standard enzyme immunoassay and latex agglutination for the detection of human rotavirus in stools. J Diarrhoeal Dis Res 1994;12:117-120)

Supplemental Report Indicates whether the report includes an additional document with charts, images or other enriched information

No

Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Monday through Sunday

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

1 day

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result

2 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

Until reported

Performing Laboratory Location The location of the laboratory that performs the test

Rochester