Test ID: FMB
Fetomaternal Bleed, Flow Cytometry, Blood
Method Description
Describes how the test is performed and provides a method-specific reference
The fetomaternal bleed test identifies cells containing fetal hemoglobin. The cells are fixed and permeabilized and then incubated with monoclonal antibodies directed against fetal hemoglobin (HbF) and subsequently analyzed by flow cytometric methods. The flow cytometry method is adapted from the Caltag package insert.(Package insert: Caltag Laboratories, Monoclonal antibodies directed to human fetal hemoglobin [HbF], P/N L13011, Rev 01-03; Davis BH, Olsen S, Biglelow NC, et al: Detection of fetal red cells in fetomaternal hemorrhage using a fetal hemoglobin monoclonal antibody by flow cytometry. Transfusion 1998;38:749-756)
RhD-Agglutination of red cells with an antiserum represents the presence of the corresponding antigen in the red cells.(Technical Manual. Sixteenth edition. Edited by J Roback, MR Combs, B Grossman, C Hillyer. Bethesda, MD, AABB Press, 2008)
Day(s) and Time(s) Test Performed
Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Sunday; Varies


