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Unit Code 8878:
D-Lactate, Plasma

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Method Description

D-lactate is oxidized to pyruvate in the presence of D-lactate

dehydrogenase and nicotinamide adenine dinucleotide phosphate

(NAD).  The reaction proceeds because the pyruvate is continually

removed as a pyruvate-hydrazone complex.  The quantity of reduced

NAD produced is directly proportional to the amount of D-lactate

oxidized and is measured spectrophotometrically at 340 nm.  (Oh MS,

Phelps KR, Traube M, et al: D-Lactic acidosis in a man with the short-

bowel syndrome.  N Engl J Med 1979;301:249-252; Dahlquist NR,

Perrault J, Callaway CW, Jones JD: D-Lactic acidosis and encephalopathy

after jejunoileostomy: response to overfeeding and to fasting in humans. 

Mayo Clin Proc 1984;59:141-145)

Performing Laboratory Location

Rochester

Key