Unit Code 8873:
D-Lactate, Urine
Method Description
D-lactate is oxidized to pyruvate in the presence of D-lactate
dehydrogenase and nicotinamide adenine dinucleotide phosphate (NAD).
The reaction proceeds because the pyruvate is continually removed as a
pyruvate-hydrazone complex. The quantity of reduced NAD produced is
directly proportional to the amount of D-lactate oxidized and is measured
spectrophotometrically at 340 nm. (Oh MS, Phelps KR, Traube M, et al:
D-Lactic acidosis in a man with the short-bowel syndrome.
N Engl J Med 1979;301:249-252)
Performing Laboratory Location
Rochester
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