Complement, Alternate Pathway (AH50), Functional, Serum
Method Description Describes how the test is performed and provides a method-specific reference
For quantitation of alternative pathway function, immunoassay strips are coated with specific activators of the alternative pathway. Patient serum is diluted in diluent containing a specific blocker to ensure that only the alternative pathway is activated. During the incubation of the diluted patient serum in the wells, complement is activated by the specific coating generating a terminal complement complex (C5b-9, membrane attack complex: MAC). Strips are washed and the MAC is detected with a specific alkaline phosphatase-labeled antibody to the neoantigen expressed during MAC formation. After additional washing, color generation is accomplished by incubation with alkaline phosphatase substrate solution. The amount of complement activation correlates with the color intensity and is measured in terms of absorbance (optical density).(Nordin JG, Truedsson L, Sjoholm A: New procedure for detection of complement deficiency by ELISA, J Imm Methods 1993;166:655-668)
PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location The location of the laboratory that performs the test