Urine proteins are separated in an electric field according to their size, shape, and electric charge (Helena SPIFE 3000). The separation is performed on agarose gels (Helena SPIFE SPE Vis GEL). The proteins are visualized by staining with acid blue and the intensity of staining is quantitated by densitometry (Helena Quickscan 2000). Multiplying by the urine protein concentration (Pyrogallol Red) converts the percentage of protein in each fraction into urine concentration.(Kyle RA, Katzmann JA, Lust JA, Dispernzieri A: Immunochemical characterization of immunoglobulins. In Manual of Clinical Laboratory Immunology. Sixth edition. Edited by NR Rose, et al. Washington, DC, ASM Press, 2002, pp 71-91)
Protein, total: Monday through Sunday; Continuously
Electrophoresis, protein: Monday through Saturday; 12 p.m.
Immunofixation: Monday through Saturday; 8 a.m.