Unit Code 8804:
C-Peptide, Serum
Method Description
The instrument used is a Roche Cobas. The Roche C-peptide assay is
a 2-site immunometric (sandwich) assay using electrochemiluminescence
detection. Patient specimen, biotinylated monoclonal C-peptide specific
antibody, and monoclonal C-peptide-specific antibody labeled with a
ruthenium react to form a complex. Streptavidin-coated microparticles act
as the solid phase to which the complex becomes bound. Voltage is
applied to the electrode inducing a chemiluminescent emission from the
ruthenium, which is then measured against a calibration curve to determine
the amount of C-peptide in the patient specimen. (Package insert: Roche
Cobas. Roche Diagnostics, Indianapolis, IN 03/2007)
Performing Laboratory Location
Rochester
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