|Values are valid only on day of printing.|
Peripheral blood mononuclear cells are isolated and stained with CD19, TACI, and BAFF-R, each conjugated to a fluorochrome. After the staining with specific antibody, the cells are washed, fixed with paraformaldehyde, and then analyzed by flow cytometry on a BD FACSCanto instrument. The cell-surface expression is denoted as the percent of CD19+ B cells expressing TACI and BAFF-R.(Unpublished Mayo method)
Specimens are required to be received in the lab weekdays and by 4 pm on Friday. No weekend processing.