Hepatitis B Surface Antibody Monitor, Post-Transplant, Serum
Method Description Describes how the test is performed and provides a method-specific reference
The ADVIA Centaur Anti-HBs2 assay is a sandwich immunoassay using direct, chemiluminometric technology. Hepatitis Bs antigen (HBsAg) (ad and ay) is coupled to magnetic latex particles in the solid phase. In the lite reagent, the HBsAg (ad and ay) is labeled with acridinium ester.
Nonmagnetic latex particles are added from the ancillary well. The sample is incubated simultaneously with lite reagent, solid phase, and ancillary reagent. Antibody-antigen complexes will form if hepatitis B surface antibody (anti-HBs) is present in the sample. Low and high anti-HBs2 calibrators are used to calibrate the ADVIA Centaur immunodiagnostic system. A direct relationship exists between the amount of anti-HBs activity present in the patient sample and the amount of relative light units (RLU) detected by the system. This assay is traceable to the WHO Hepatitis B Immunoglobulin First International Reference Preparation (1977), and quantitative anti-HBs result is expressed in mIU/mL.(Package insert: ADVIA Centaur Anti-HBs2; Siemens Healthcare Diagnostics Inc., Tarrytown, NY. Publication no. 10698735_EN Rev. A, 2012-02)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Saturday; Varies
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location The location of the laboratory that performs the test