Mobile Site ›
Print Page Email Page
Print Friendly View

Test ID: FNMYC    
Neuroblastoma, 2p24 (MYCN) Amplification, FISH

Method Description Describes how the test is performed and provides a method-specific reference

The test uses a dual-color FISH probe strategy with an MYCN probe and a chromosome 2 alpha-satellite centromere probe (D2Z1) (Abbott Molecular). Paraffin-embedded tissues are cut at 5 microns and mounted on positively charged glass slides. Four slides are prepared, with 1 slide stained with hematoxylin-and-eosin (H and E). The selection of tissue and the identification of target areas on the H and E-stained slide is performed by a pathologist. Using the H and E slide as a reference, target areas are etched with a diamond-tipped etcher on the back of the unstained slide to be assayed. The probe set is hybridized to the appropriate target areas and 2 technologists analyze 30 interphase nuclei each (100-300 cells) with the results expressed as a ratio of the total number of MYCN signals compared to the total number of D2Z1 signals. (Unpublished Mayo method)

Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Samples processed Monday through Sunday. Results reported Monday through Friday, 8 a.m.-5 p.m. CST.

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

7 days

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result

10 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

Indefinitely

Performing Laboratory Location The location of the laboratory that performs the test

Rochester