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Unit Code 87843:
Macroprolactin, Serum

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Method Description

The instrument use for testing is the Roche Cobas 6000. An equal

volume of 25% polyethylene glycol (PEG) is added to a serum

prolactin sample causing the biologically inactive form of prolactin

(macroprolactin) to precipitate. Following centrifugation, prolactin

is measured in the supernatant (containing unprecipitated prolactin),

as well as in an aliquot of the native sample. Precipitated prolactin

is calculated by subtracting the unprecipitated prolactin value from

the total prolactin. The percentage of precipitated prolactin to total

prolactin indicated the presence or absence of macroprolactin.

(Package insert: Elecsys and Modular Prolactin II, Roche 2005)

Performing Laboratory Location

Rochester

Key