Cyclic AMP, Urinary Excretion
Method Description Describes how the test is performed and provides a method-specific reference
Adenosine cyclic 3',5'-monophosphate (cyclic AMP) is isolated from the urine by using a standard anion exchange column (Dowex-1 in formate form). An internal standard (8-methylamino cyclic AMP) is used to correct for recovery losses. Once the cyclic AMP has been eluted from the column, it is allowed to react under acidic conditions with chloracetaldehyde to give a flourescent 1, N(6)-ethano derivative of cyclic AMP. The modified nucleotides are excited at 316 nm and the emission is measured at 410 nm in a fluorometric detector. Quantitation is by peak height measurement against a calibration standard. Urine and serum creatinine levels are used to determine the clearance of cyclic AMP from the kidneys. (Aurbach GD, Marx SJ, Spiegel AM: Parathyroid hormone, calcitonin, and the calciferols. In Williams Textbook of Endocrinology. 8th edition. Edited by JD Wilson, DW Foster. Philadelphia, WB Saunders Company, 1992, pp 1413-1415)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Wednesday; 9 a.m.
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location The location of the laboratory that performs the test