Specimens are plated on mycobiotic agar, which contains chloramphenicol and cyclohexamide to inhibit bacterial and saprobic fungal contamination. Cultures are incubated at 30 degrees C for 30 days. Identification of dermatophyte species is based on colony and microscopic morphology and PCR, DNA sequencing or MALDI-TOF mass spectrometry, when applicable.(Hall L, Wohlfiel S, Roberts GD: Experience with the MicroSeq D2 large-subunit ribosomal DNA sequencing kit for identification of filamentous fungi encountered in the clinical laboratory. J Clin Microbiol 2004;42:622-626); (Theel ES, Hall L, Mandrekar J, et al., Dermatophyte identification using matrix-assisted lazer desorption ionization-time of flight mass spectrometry. J Clin Microbiol 2011; 49:4067-4071)
Positive cultures reported when detected. Negative cultures reported after 30 days.