Test ID: VIRNR
Viral Culture, Non-Respiratory
Method Description
Describes how the test is performed and provides a method-specific reference
Specimens are inoculated into conventional tube cell cultures (MRC-5 and RMK) and observed for the presence of cytopathic effects (CPE) over a 14-day period. Results of viral cultures are reported when typical CPE are detected and the identity of the isolate has been confirmed by immunofluorescence with specific antisera. (Smith TF: Antibody-enhanced detection of viruses in cell cultures. In Manual of Clinical Laboratory Immunology. 5th edition. Edited by NR Rose, EC de Macario, JD Folds, et al. Washington, DC, ASM Press, 1997, pp 618-624)
The shell-vial assay is also performed on specimens submitted for cytomegalovirus or herpes simplex virus. Shell-vial results may be positive 16 to 24 hours postinoculation. (Paya CV, Wold AD, Smith TF: Detection of cytomegalovirus infections in specimens other than urine by the shell vial assay and conventional tube cell cultures. J Clin Microbiol 1987;25:755-757)
Day(s) and Time(s) Test Performed
Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Sunday; Varies


