|Values are valid only on day of printing.|
Lactate and NAD+, in the presence of LD, are converted to pyruvate and NADH. The rate at which NADH is formed is determined by an increase in absorbance and is directly proportional to enzyme activity.(Package insert: Roche LDH reagent, Indianapolis, IN, November, 1999)
The 5 isoenzymes of LD are separated by electrophoresis on agarose film. The serum samples are electrophoresed and separated LD isoenzymes are visualized using a specific chromogenic substrate. Densitometry is used to obtain relative quantification of each fraction. The fractions are numbered according to their electrophoretic mobility, LD-I being the most mobile.(Clinical Guide to Laboratory Tests. Third edition. Edited by NW Tietz, Philadelphia, WB Saunders Company,1995)
LD: Monday through Sunday; Continuously
LD isoenzymes: Tuesday, Thursday, Saturday; 10 a.m.