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Unit Code 8665:
Lactate, Plasma

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Method Description

The instrument used is a Vitros 250. Lactate in the specimen is

oxidized by lactate oxidase to pyruvate and hydrogen peroxide.

The generated hydrogen peroxide oxidizes the

4-aminoantipyrine, 1,7-dihydroxynaphthlaene dye system in a

horseradish peroxidase-catalyzed reaction and results in a dye

complex. Each mole of lactate oxidized results in 0.5 mole of the

dye complex. The specimen is incubated at 37 degrees C for 5

minutes and the intensity of the dye complex is measured

spectrophotometrically at  540 nm. (Package Insert: Vitros 250.

Ortho-Clinical Diagnostics, Inc., Rochester, NY, 2004)

Performing Laboratory Location

Rochester

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