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The internal standard mixture containing chlordiazepoxide-d5, diazepam-d4, and nordiazepam-d5 is added to serum samples The serum samples are treated with 0.1M phosphate buffer and extracted via liquid/liquid extraction with 50/50 (hexane/ethyl acetate). The organic layer from the extraction is dried under nitrogen, reconstituted in 95/5 (H2O/Methanol) and injected on a liquid chromatography-tandem mass spectrometer (LC-MS/MS).(Unpublished Mayo method)
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