|Values are valid only on day of printing.|
A buffered enzyme incubation with substrate and cofactors is performed on lysed red blood cells. A postincubation extraction is performed and subjected to liquid chromatography-tandem mass spectrometry. The ratio of the extracted product to its internal standard is used to calculate the total enzymatic product. This is then normalized using the calculated hemoglobin concentration to determine the patient's enzyme level in nmol/h/mg of hemoglobin.(Unpublished Mayo method)
Mondays; 9 a.m.