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Unit Code 8628:
Galactokinase, Blood

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Method Description

Galactokinase is determined by using red blood cells (as is

GPUT).  Radiolabeled galactose is incubated in the appropriate

buffer with adenosine triphosphate (ATP) and red cell lysate.  

Galactose-1-phosphate (the product of the action of galactokinase

on galactose and ATP) is quantitated after it is bound to ion-exchange

chromatography paper. (Beutler E, Paniker NV,Trinidad F:  The

assay of red cell galactokinase.  Biochem Med 1971;5:325-332)  

Performing Laboratory Location

Rochester

Key