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Test ID: AB2GP    
Beta-2 Glycoprotein 1 Antibodies, IgA, Serum

Method Description Describes how the test is performed and provides a method-specific reference

This indirect, noncompetitive enzyme-linked immunosorbent assay (ELISA) uses microtiter plates coated with purified, human beta 2 GP1. Beta 2 GP1 antibodies bound in the first stage of the assay are detected with antihuman IgA horseradish peroxidase antibody conjugate in the second stage of the assay by adding 3,3',5,5' tetramethylbenzidine substrate (10 minute incubation followed by addition of 0.5 M H[2]SO[4]). The absorbance of test sera is compared to a single cutoff calibrator. Results for test sera are expressed in arbitrary U/mL by comparison with standard curves obtained using calibrators at 0, 4, 8, 20, 50, and 100 U/mL. (Package insert: Varelisa Beta 2 Glycoprotein 1 IgM, Antibodies, doc 189-01, Phadia Diagnostics, Portage, MI)

Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Monday through Saturday; 8 a.m.

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

Same day/1 day

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result

6 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

7 days

Performing Laboratory Location The location of the laboratory that performs the test

Rochester