Thiamin (Vitamin B1), Whole Blood
Method Description Describes how the test is performed and provides a method-specific reference
An aliquot of whole blood is slowly mixed with methanol to precipitate the proteins. Following centrifugation, an aliquot of the clarified supernatant is derivatized using alkaline potassium ferricyanide. The derivatized solution is then subjected to high-performance liquid chromatography, during which derivatized thiamin diphosphate is separated from other components. Quantitation is achieved by fluorometry. The fluorescence characteristics of the thiochrome derivative, together with its unique chromatographic mobility, provide a high level of specificity for this method.(Lynch PL, Young IS: Determination of thiamine by high-performance liquid chromatography. J Chromatogr A 2000;881:267-284; Mancinelli R, Ceccanti M, Guiducci MS, et al: Simultaneous liquid chromatographic assessment of thiamine, thiamine monophosphate and thiamine diphosphate in human erythrocytes: a study in alcoholics. J Chromatogr B 2003;789:355-363)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Friday
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
2 days (not reported Saturday or Sunday)
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location The location of the laboratory that performs the test