Following chromatographic purification, which results in a highly purified and concentrated final eluate, the purified amines are quantitated by using high-performance liquid chromatography with electrochemical detection.(Jiang NS, Machacek D, Wadel OP: Further study on the two-column plasma catecholamine assay. Mayo Clin Proc 1976;51:112-116; Jiang NS, Machacek D: Measurement of catecholamines in blood and urine by liquid chromatography with amperometric detection. In Progress in HPLC. Vol 2. Edited by Parvez. VNU Science Press, 1987, pp 397-426)
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