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Unit Code 8522:
Acetylcholinesterase, Erythrocytes

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Method Description

The substrate acetylthiocholine is split by acetylcholinesterase

into thiocholine and acetate.  The thiocholine then reacts with

dithiobisnitro benzoic acid (Ellmans Reagent) to form the colored

product thionitrobenzoic acid which is measured at 405 nm.

(Ellman GL, Courtney KD, Andres V Jr, Featherstone RM:  A new

and rapid colorimetric determination of acetylcholinesterase activity. 

Biochem Pharmacol 1961;7:88-95)

Performing Laboratory Location

Rochester

Key