Mumps Virus Antibody, IgG and IgM (Separate Determinations), Serum
Method Description Describes how the test is performed and provides a method-specific reference
The SeraQuest mumps IgG test is an ELISA test. Diluted samples are incubated in mumps (Enders strain) antigen-coated wells. If present, mumps antibodies are immobilized in the wells. Wells are washed, removing excess sample. Goat anti-human IgG (labeled with alkaline phosphatase) conjugate is added and the wells are incubated. If IgG antibodies to mumps are present, the conjugate will be immobilized in the wells. Wells are washed, removing excess conjugate. Substrate (p-nitrophenyl phosphate) is added and wells are incubated. If the enzyme conjugate is present, the substrate is converted to a yellow end product which is read photometrically. (Package insert: Mumps IgG, SeraQuest, 1938 NE 148th Terrace, North Miami, FL 33181)
The SeraQuest mumps IgM is an enzyme capture method. Diluted samples are incubated in wells coated with anti-human IgM monoclonal antibodies. If present, IgM antibodies are captured in the wells. Wells are washed, removing excess sample. Conjugate-antigen complex (mumps antigen in a complex with monoclonal antibodies conjugated to horseradish peroxidase) is added and wells are incubated. IgM antibodies specific for the antigen will bind the conjugate. Wells are washed, removing excess conjugate. Peroxidase substrate is added and wells are incubated. Stop solution is added converting the substrate to a yellow end product which is read photometrically. (Package insert: Mumps IgG, SeraQuest, 1938 NE 148th Terrace, North Miami, FL 33181)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Mumps Virus Antibody, IgG: Monday through Saturday; 9 a.m.
Mumps Virus Antibody, IgM: Monday through Friday; 4 p.m.
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
Same day/1 day
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location The location of the laboratory that performs the test