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Unit Code 8512:
Gastrin, Serum

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Method Description

The IMMULITE 2000 gastrin is a solid-phase, chemiluminescent

immunoassay. The patient sample and alkaline phosphatase-

conjugated antigastrin antibodies and biotin-labeled antigastrin

antibody are simultaneously incubated with streptavidin-coated

beads. During this time, gastrin in the sample forms an antibody

sandwich complex that binds to the streptavidin on the beads.

Unbound enzyme conjugate is then removed by washing, after

which substrate is added. The chemiluminescent substrate, a

phosphate ester of adamantyl dioxetane, undergoes hydrolysis

in the presence of alkaline phosphatase to yield an unstable

intermediate. The continuous production of this intermediate results

in the sustained emission of light. The photon output is directly

proportional to the concentration of gastrin in the sample. (Package

insert: IMMULITE 2000 Gastrin [PIL2KGA-10, 2006-12-29])

Performing Laboratory Location

Rochester

Key