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Unit Code 8493:
Dehydroepiandrosterone Sulfate (DHEA-S), Serum

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Method Description

The IMMUNLITE 2000 DHEA-SO(4) is a solid-phase, chemiluminescent

immunoassay. A polystyrene bead is coated with a polyclonal rabbit

antibody specific for DHEA-SO(4). The patient sample and alkaline

phosphatase-conjugated DHEA-SO(4) are added to the bead and

incubated during this time, DHEA-SO(4) in the sample competes

with the enzyme-labeled DHEA-SO(4) for a limited number of antibody

binding on the bead. Unbound enzyme conjugate is then removed

by washing, after which substrate is added. The chemiluminescent

substrate, a phosphate ester of adamantyl dioxetane, undergoes

hydrolysis in the presence of alkaline phosphatase to yield an

unstable intermediate. The continuous production of this intermediate

results in the sustained emission of light. The photon output, is inversely

proportional to the concentration of DHEA-SO(4) in the sample.

(Package insert: IMMUNLITE  2000 DHEA-SO(4) [PIL2KDS-7;

002-04-08] Diagnostics Products Corporation)

Performing Laboratory Location

Rochester

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