Test ID: FIBR
Fibroblast Culture
Method Description
Describes how the test is performed and provides a method-specific reference
Submitted biopsy specimens are minced, placed in a culture flask with additional culture media supplemented with fetal calf serum, and incubated. When the fibroblasts have grown from the pieces of tissue (2-4 weeks), the cultures are expanded and then, if indicated, cryopreserved (CRYOB/88832 Cryopreservation for Biochemical Studies). If specific molecular or biochemical testing is concurrently ordered, cells will be harvested or prepared for indicated testing. Submitted cultured cells are examined for cell integrity and fed fresh media. Once grown to confluency, the cultures are expanded and then, if indicated, cryopreserved (CRYOB/88832 Cryopreservation for Biochemical Studies). If specific molecular or biochemical testing is concurrently ordered, cells will be harvested or prepared for indicated testing.
Day(s) and Time(s) Test Performed
Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Varies


