Prolactin, Pituitary Macroadenoma, Serum
Method Description Describes how the test is performed and provides a method-specific reference
This assay is performed on the Beckman Coulter UniCel DXI 800 instrument using the Access Prolactin assay, a simultaneous 1-step immunoenzymatic sandwich assay. Sample is added to a reaction vessel along with polyclonal goat anti-prolactin (anti-PRL) alkaline phosphatase conjugate, and paramagnetic particles coated with mouse monoclonal anti-PRL antibody. Prolactin in the sample binds to the monoclonal anti-PRL on the solid phase, while the goat anti-PRL-alkaline phosphatase conjugate reacts with a different antigenic site on the sample prolactin. After incubation in a reaction vessel, materials bound to the solid phase are held in a magnetic field, while unbound materials are washed away. The chemiluminescent substrate Lumi-Phos 530 is added to the vessel and light generated by the reaction is measured with a luminometer. The light production is directly proportional to the concentration of prolactin in the sample. The amount of analyte in the sample is determined from a stored, multipoint calibration curve. Serial dilutions are prepared and analyzed, in addition to the neat sample, and results are evaluated for linearity. Non-linear results are forwarded to lab director for review and comment. (Assay manual: Beckman Coulter Inc. Fullerton CA, 2010)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Friday; 5 a.m.-5 p.m., Saturday; 6 a.m.-6 p.m.
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
Same day/1 day
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location The location of the laboratory that performs the test