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Test ID: RHIV    
HIV Antibody Rapid Test Confirmatory Profile, Serum

Method Description Describes how the test is performed and provides a method-specific reference

HIV 1 and 2 Antibody Screen:

HIV-1/-2 screening chemiluminescent immunoassay: The VITROS Anti-HIV 1+2 assay is performed using the VITROS Immunodiagnostic Products Anti-HIV 1+2 Reagent Pack and the VITROS Immunodiagnostic Products Anti-HIV 1+2 Calibrator on the VITROS ECi/ECiQ Immunodiagnostic System. An immunometric bridging technique is used, involving a 2-stage reaction. In the first stage, HIV antibody present in the sample binds with HIV recombinant antigen coated on the wells. Unbound sample is removed by washing. In the second stage, horseradish peroxidase (HRP)-labeled recombinant HIV antigens are added in the conjugate reagent. The conjugate binds specifically to any human anti-HIV-1 or anti-HIV-2 (IgG and IgM) captured on the well in the first stage. Unbound conjugate is removed by washing. The bound HRP conjugate is measured by a luminescent reaction. A reagent containing luminogenic substrates (a luminol derivative and a peracid salt) and an electron transfer agent is added to the wells. The HRP in the bound conjugate catalyzes the oxidation of the luminol derivative, producing light. The electron transfer agent (a substituted acetanilide) increases the level of light produced and prolongs its emission. The light signals are read by the VITROS ECi/ECiQ Immunodiagnostic System. The amount of HRP conjugate bound is indicative of the level of anti-HIV-1 or anti-HIV-2 present.(Package insert: VITROS Anti-HIV 1+2, Ortho Clinical Diagnostics Inc., Raritan, NJ; publication no. GEM1252_EN)

 

HIV 1 and 2 Antibody Confirmatory Evaluation:

Samples are diluted and applied to the nitrocellulose strip containing specific protein bands derived from purified, inactivated HIV-1 strain lymphadenopathy associated virus (LAV) grown in the CEM cell line. If specific HIV-1 antibodies are present, they bind to these proteins resolved on the strip. Unbound sample is removed by washing. The phosphatase-labeled conjugate is then added to the strip and allowed to incubate. The conjugate attaches to antibody already bound to viral proteins on the strip. Excess conjugate is removed by washing. Color development reagent is then added to the strip. Reaction sites, where enzyme-labeled antibody is bound, are identified by dark bands. The position and intensity of the bands are compared to reference strips developed using positive control sera. All specimens yielding indeterminate or uninterpretable results are retested, and results are reported based on the best 2 out of 3 replicate testing.(Package insert: Genetic Systems HIV-1 Western blot. Bio-Rad Laboratories, Redmond, WA)

Supplemental Report Indicates whether the report includes an additional document with charts, images or other enriched information

No

Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

HIV-1/-2 antibody screen: Monday through Saturday

HIV-1/-2 antibody confirmatory evaluation: Monday through Friday

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

Same day/1 day

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result

3 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

7 days

Performing Laboratory Location The location of the laboratory that performs the test

Rochester