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Test ID: ROP    
Rubeola (Measles) Antibodies, IgG and IgM (Separate Determinations), Serum

Method Description Describes how the test is performed and provides a method-specific reference

The presence or absence of IgG class antibody to rubeola is determined by an enzyme-linked immunosorbent assay (ELISA).

 

In the Immunosimplicity (IS) Measles IgG ELISA test, diluted specimens are incubated with measles antigen bound to the solid surface of a microtiter well. If IgG antibodies against measles are present in the specimen, they will bind to the antigen forming antigen-antibody complexes. Residual specimen is eliminated by aspiration and washing. Conjugate (horseradish peroxidase-labeled antihuman IgG) is added and will bind to these complexes. Unbound conjugate is removed by aspiration and washing. Substrate is then added and incubated. In the presence of bound enzyme, the substrate is converted to an end product. The absorbance of this end product can be read spectrophotometrically at 450 nm (reference 600-630 nm) and is directly proportional to the concentration of IgG antibodies to measles antigen present in the specimen. (Package insert: Immunosimplicity [Is] Measles IgG Enzyme Immunoassay Test Kit. Diamedix Corporation, Miami, FL; Herrman KL: Antibody detection. In Laboratory Diagnosis of Infectious Diseases Principles and Practice. Edited by EH Lennette, P Halonen, FA Murphy. New York, Springer-Verlag, 1988, pp 76-101)

 

The presence of IgM class antibody to rubeola is determined by an immunofluorescence assay (IFA). After removal of IgG by specific immunoglobulin antibody, the serum is reacted with rubeola- infected substrate cells on a slide. Specific fluorescence indicating the presence of IgM antibodies to rubeola in the specimen can be detected by an immunofluorescence microscope. (Package insert: Measles Virus Antigen Substrate Slide, BION Enterprises, Des Plaines IL)

Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Monday through Friday; 9 a.m. and 3 p.m., Saturday, Sunday; Varies

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

Same day/1 day

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result

2 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

14 days

Performing Laboratory Location The location of the laboratory that performs the test

Rochester