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Test ID: SCMV    
Cytomegalovirus (CMV) Antibodies, IgG and IgM, Serum

Method Description Describes how the test is performed and provides a method-specific reference

VIDAS cytomegalovirus (CMV) IgG and CMV IgM are enzyme-linked fluorescence immunoassays performed by an automated VIDAS instrument. All assay steps are controlled by the instrument.

 

IgG:

In the test, CMV IgG antibodies bind to CMV antigen coated on a solid-phase pipetter. Antihuman IgG antibody conjugated with alkaline phosphatase bind to specific antigen-antibody complexes. A fluorescent substrate, 4-methylumbelliferyl phosphate, is added. In the presence of bound enzyme, the substrate is converted to the fluorescent product, 4-methylumbelliferone. The intensity of fluorescence is measured by the optical scanner in the VIDAS instrument. (Package insert: VIDAS CMV IgG, bioMerieux, Inc., Durham, NC)

 

IgM:

IgG and rheumatoid factor are absorbed from the serum specimen. Anti-CMV IgM antibodies present in the specimen bind to CMV antigen (strainAD 169) coating the interior of a solid-phase receptacle (SPR). Unbound specimen components are washed away. Mouse monoclonal antihuman IgM antibodies conjugated with alkaline phosphatase are cycled in and out of the SPR and attach to human anti-CMV bound to the SPR wall. A fluorescent substrate, 4-methylumbelliferyl phosphate, is introduced into the SPR. Enzyme remaining on the wall of the SPR will catalyze the conversion of the substrate to the fluorescent product, 4-methylumbelliferone. The intensity of the fluorescence is measured by the optical scanner in the instrument. (Package insert: VIDAS CMV IgM, bioMerieux, Durham, NC)

Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Monday through Saturday; Varies

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

Same day/1 day

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result

3 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

14 days

Performing Laboratory Location The location of the laboratory that performs the test

Rochester