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Unit Code 84406:
Vancomycin-Resistant Enterococcus (VRE) Detection by Rapid PCR

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Method Description

Bacterial DNA is extracted from the specimen using the

automated MagNA Pure instrument. Using the LightCycler

instrument and fluorescent energy transfer probes, vanA

and vanB genes responsible for vancomycin resistance

in enterococci are detected directly from perianal swabs or

fecal specimens. (Sloan LM, Uhl JR, Vetter EA, et al: Comparison of the

Roche LightCycler vanA/vanB detection assay and culture for detection

of vancomycin-resistant enterococci from perianal swabs. Submitted

J Clin Microbiol 2004;42:2636-2643)

Performing Laboratory Location

Rochester

Key