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A polymerase chain reaction (PCR)-based assay utilizing
fluorescence resonance energy transfer (FRET) probes,
melt-curve analysis, and LightCycler technology is used to
examine the GALT gene for the presence of the known familial
mutation(s). (Wittwer CT, Herrmann MG, Gundry CN, Elenitoba-
Johnson KS: Real-time multiplex PCR assays. Methods 2001
Dec;25[4]:430-442)

